Indirect auxiliary organogenesis of Fraxinus excelsior L. as a tool for ash dieback control

Abstract The existence of European ash ( Fraxinus excelsior L.) is threatened by fungus-induced dieback. It essential to find effective methods multiply genotypes resistant dieback while preserving the genetic diversity these tree populations. In this paper efficient method for production seedlings using indirect auxiliary organogenesis with multi-factor analysis its effectiveness presented. Procedures a dormancy breaking treatment seeds and disinfection F. primary explants, as well appropriate composition culture media taking into account impact growth regulators physiological gradient on micropropagation efficiency were developed. As explant , leaf buds, megagametophytes zygotic embryos tested. best-performing type proved be embryos, which successfully used regenerate via organogenesis. No statistically significant population origin donor trees was observed callus initiation. However, such difference in regard average productivity acquired cultures (number produced) root length regenerated seedlings. Health condition their affects initiation rate from but does not affect lines derived quality Indirect developed our study, only provides acquisition different genotypes, also enables rapid selection desired already at stage. way, presented benefits profit oriented forestry wood industry, provide fully controllable tool reintroduction various ash-dieback without loss variability identity